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Bioremediation, Heavy metals, Bioinformatics

The present research work has been carried out to study the waste disposal contaminated site for its physico chemical and microbial characterization and identification of potential microorganism capable of bioaccumulation and biodegradation of heavy metals. The ambient conditions present in the metal contaminated environment shows the values: pH(5.4),temperature(30°C), moisture(11.71%), nutrients; Nitrogen(0.2mg/l), phosphorus(22.65mg/l) and sulphur(559.3mg/l) respectively. The biological parameters studied indicate Dissolved oxygen (7.4mg/l), Biological oxygen demand (3.8 mg/l), Chemical oxygen demand (64.6 mg/l). The microbial consortium identified was found to survive and multiply in the present environmental conditions. Microbial consortium was sequenced and compared using Bioinformatics tools like BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentrations of heavy metals viz 5mg/l, 25mg/l, 50mg/l, 100mg/l up to 800mg/l with special reference to Iron. At a concentration of 500mg/l, only one microorganism was found survived and multiplied. This shows that potential microorganism was only survived at higher concentration of iron. The 16SrRNA sequence and phylogenetic tree characterized the organism as Klebsiella pneumoniae, which was also confirmed by biochemical tests. The potential microorganism identified by BLAST technique can be used for remediation of the heavy metal from contaminated environment.